Human Aldolase C, Fructose Bisphosphate (ALDOC) ELISA Kit

Calibrated against a seven-point standard curve spanning 0.78 to 50 ng/mL, this Assay Genie kit reports sensitivity typically below 0.25 ng/mL. Validation data lists intra-assay precision CV less than 10% and inter-assay precision CV less than 12%, with a loss rate below 5% over the shelf life.

Spike-and-recovery performance in human matrices yields 80-102% in serum (average 91%), 81-100% in EDTA plasma (average 90%), and 80-89% in heparin plasma (average 84%). Parallelism is confirmed across serial 1:2, 1:4, 1:8, and 1:16 sample dilutions. Specificity testing showed no significant cross-reactivity with aldolase analogues such as ALDOA or ALDOB.

Reagents include a lyophilized standard in 2 vials, 45 mL of diluent buffer, 120 µL each of detection reagents A and B, 9 mL of TMB substrate, 6 mL of stop solution, and 20 mL of 30× wash buffer, plus 2 plate sealers. The workflow uses 100 µL of standard or sample per well with 2-hour and 1-hour incubations at 37 °C, a 15 to 25 minute protected-from-light substrate step, and a final spectrophotometric read. Store unopened components at -20 °C with a 12-month shelf life; the TMB substrate, wash buffer, and stop solution are kept refrigerated upon receipt.