Human Aldo-keto reductase family 1 member B10/AKR1B10 ELISA Kit

Assay Genie's Human Aldo-keto reductase family 1 member B10 (AKR1B10) ELISA Kit is intended for the quantitative measurement of AKR1B10 in human samples. The kit uses a sandwich ELISA, double-antibody format and accepts a broad range of sample types including serum, plasma, cell culture supernatant, cell or tissue lysates, and other liquid samples. The reported sensitivity is 0.469 ng/mL with a working range of 0.781-50 ng/mL. All components should be stored at 2-8°C, where the kit remains stable for up to 12 months.

Validation data supplied by Assay Genie include linearity and recovery testing across serum, EDTA plasma and heparin plasma (n=5 per matrix). Linearity across 1:2 to 1:8 dilutions falls within 82-119%, while recovery ranges between 88-103% depending on the sample matrix. The procedure uses a pre-coated plate, a biotin-labelled detection antibody, HRP-Streptavidin (SABC) and TMB substrate, with absorbance read at 450 nm after the stop step. The kit is positioned within the cell biology research area, supporting studies of aldo-keto reductase family members and their roles in metabolic and disease-related research.