Human ALDH1A1/Retinal dehydrogenase 1 ELISA Kit

This Sandwich ELISA, Double Antibody kit from Assay Genie is designed for the quantitative measurement of human ALDH1A1 (Retinal Dehydrogenase 1) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay uses a colorimetric detection method with absorbance read at 450 nm, and the manufacturer cites a sensitivity of 0.938 mIU/mL across a range of 1.563-100 mIU/mL. ALDH1A1 is an enzyme involved in retinoic acid metabolism, and its dysregulation has been linked to cancer, cardiovascular disorders, and neurodegenerative conditions, supporting its use as a biomarker in molecular biology research.

Performance characteristics reported by the manufacturer include linearity for serum (98-105%, 85-105%, 91-104%), EDTA plasma (87-98%, 85-99%, 85-98%), and heparin plasma (83-93%, 85-100%, 84-99%) at serial dilutions. Recovery is reported as 93-96% (average 94%) for serum, 86-97% (average 90%) for EDTA plasma, and 87-104% (average 95%) for heparin plasma. The procedure involves equilibrating reagents, incubating standards and samples at 37°C for 90 minutes, adding biotin-labelled detection antibody for 60 minutes at 37°C, adding HRP-Streptavidin for 30 minutes at 37°C, developing with TMB substrate for 10-20 minutes in the dark, and reading absorbance at 450 nm after adding stop solution.

Kit components include a dismountable ELISA microplate, lyophilised standard, biotin-labelled antibody (100X), HRP-Streptavidin conjugate (SABC, 100X), TMB substrate, sample dilution buffer, antibody dilution buffer, SABC dilution buffer, stop solution, 25X wash buffer, plate sealer, and a technical manual. Storage conditions specified are 2-8°C for 12 months for the kit as a whole, with selected components also storable at -20°C.