Human Alcohol Dehydrogenase 1 (ADH1) ELISA Kit

Quantify human Alcohol Dehydrogenase 1 (ADH1) protein levels with this quantitative immunoassay kit from Assay Genie. ADH1 is a zinc-dependent cytosolic enzyme that catalyzes the NAD⁺-linked oxidation of ethanol to acetaldehyde, predominantly expressed in hepatocytes, where it governs the rate-limiting first step of hepatic alcohol clearance. The kit supports research into hepatic ethanol metabolism, alcohol-induced liver injury, acetaldehyde toxicity, and ADH1B/ADH1C pharmacogenomic variation. Validated for human serum specimens, plasma specimens, and tissue homogenate preparations, the assay is supplied as a complete reagent set sufficient for 96 determinations in a microplate format. Standard curve-based quantitation enables comparison of ADH1 concentrations across experimental groups, with the enzymatic reaction read on a standard microplate reader. Each kit package includes a pre-coated plate, assay diluent, detection antibody, wash buffer, substrate solution, stop reagent, and a recombinant human ADH1 protein standard for curve generation. Intended for research use only in laboratory settings investigating metabolic enzyme expression, oxidative stress pathways, and biomarker discovery in alcohol-related disease models.