Human Albumin PharmaGenie ELISA Kit (SBRS0241)

The Assay Genie Human Albumin PharmaGenie ELISA Kit enables quantitative measurement of circulating human serum albumin, the most abundant plasma protein in mammals. Synthesized by hepatocytes and a long-established biomarker for hepatic and renal function, albumin supports oncotic pressure and transports hormones, fatty acids, and drugs through the bloodstream.

Investigators in hepatology, nephrology, and critical care research apply this microplate-based immunoassay to characterize hypoalbuminemia in cirrhosis, nephrotic syndrome, severe burns, sepsis, and chronic malnutrition. The procedure relies on a pre-coated capture antibody, a biotinylated detection reagent, and streptavidin-HRP for chromogenic development measured on a standard plate reader. Reconstituted standards and detection antibody should be aliquoted to limit freeze-thaw degradation and stored according to the supplied insert.

The format accepts blood-derived matrices and conditioned cell culture supernatant, pipetted in duplicate into the supplied well plate for analysis. A calibrator series spanning physiologically relevant concentrations allows interpolation of unknowns, supporting biomarker discovery, pharmacokinetic studies of albumin-bound therapeutics, and translational research on protein turnover in inflammation and critical illness. Intended for in vitro research use only.