Human AKR1C3 (Aldo-keto reductase family 1 member C3) ELISA Kit (HUFI03431)

This Assay Genie immunoassay targets AKR1C3, a cytosolic NADPH-dependent oxidoreductase that catalyzes the conversion of androstenedione to testosterone, delta-4-androstane-3,17-dione to 5alpha-dihydrotestosterone, and estrone to 17beta-estradiol. The enzyme also reduces prostaglandin D2 to 11-beta-prostaglandin F2-alpha and participates in bile acid and xenobiotic metabolism. Because AKR1C3 is over-expressed in castration-resistant prostate tumors, ER-positive breast carcinomas, and endometriotic lesions, circulating and tissue-associated levels of the protein are studied as biomarkers of steroid-driven disease progression and as indicators of response to AKR1C3 inhibitors under development for oncology. The kit includes a pre-coated microplate, biotinylated detection antibody, standards, and streptavidin-HRP reagents intended for in vitro research use only; researchers should aliquot reagents and avoid repeated freeze-thaw cycles to preserve activity. Quantitation is performed by interpolation against a standard curve, supporting studies of hormone metabolism, drug metabolism, and prostaglandin signaling pathways.