Human AKAP1 ELISA Kit (HUFI04651)

This quantitative immunoassay from Assay Genie measures A-kinase anchoring protein 1 (AKAP1), a mitochondrial outer-membrane scaffold that anchors protein kinase A to organelles governing oxidative phosphorylation, apoptosis, and reactive oxygen species signaling. The 96 Assays configuration supports research into mitochondrial dysfunction in cardiomyocytes, metabolic syndrome, and tumor microenvironments where altered AKAP1 expression has been reported. Capture antibodies are pre-coated onto a microplate, with bound analyte detected using a biotinylated secondary antibody and streptavidin-HRP reagent, producing absorbance proportional to AKAP1 concentration. The protocol recommends a 100 ul sample volume per well, and all reagents are supplied ready-to-use or as concentrated stocks to minimize preparation time. For research use only, not intended for diagnostic procedures.