Human AhR (Aryl Hydrocarbon Receptor) CLIA Kit (HUES00360)

Assay Genie's sandwich CLIA kit enables in vitro quantitative determination of human aryl hydrocarbon receptor (AhR) in serum, plasma and other biological fluids using a chemiluminescence-based detection method. The assay is performed on a microplate pre-coated with an antibody specific to human AhR; standards or samples are combined with the capture antibody, followed by sequential incubation with a biotinylated detection antibody and an Avidin-Horseradish Peroxidase (HRP) conjugate. After washing, a substrate solution is added and the relative light unit (RLU) value is measured on a chemiluminescence immunoassay analyzer, with signal positively correlated to AhR concentration.

The kit recognizes human AhR in samples, with no significant cross-reactivity or interference between human AhR and analogues reported. Sensitivity is 46.88 pg/mL with a detection range of 78.13-5000 pg/mL, total assay time of 4.5 hours, and a 100 µL sample volume. Reproducibility is reported with both intra-assay and inter-assay coefficients of variation below 15%. The kit is supplied with a CLIA plate, reference standard, concentrated biotinylated detection antibody (100×), concentrated HRP conjugate (100×), reference standard and sample diluent, biotinylated detection antibody diluent, HRP conjugate diluent, concentrated wash buffer (25×), substrate reagents A and B, desiccant, plate sealer, and product documentation. Storage conditions vary by component, including -20°C for the CLIA plate, reference standard, detection antibody, and HRP conjugate (the latter two protected from light), and 4°C for the diluents and substrate reagents.

Recovery has been evaluated in serum, EDTA plasma, and cell culture media, with average recovery values of 104%, 107%, and 105% respectively. Linearity has been demonstrated across dilution factors from 1:2 to 1:16 in serum, EDTA plasma, and cell culture media. The kit supports cell biology research, providing a tool for the accurate detection of aryl hydrocarbon receptor levels in human biological samples.