Human AFAP1 (Actin Filament Associated Protein 1) ELISA Kit (HUES01521)

The Assay Genie Human AFAP1 Sandwich ELISA Kit is configured as a 96-well microplate assay supporting the quantitative detection of Actin Filament Associated Protein 1 in human serum, plasma, and other biological fluids. The kit uses a colorimetric readout and is intended for research use only, supporting studies into cytoskeletal remodeling, Src-family kinase signaling, and AFAP1-associated oncogenic pathways in breast, prostate, and head-and-neck cancer models.

Each kit provides pre-coated wells, standards, detection antibodies, and assay buffer sufficient for 96 determinations. Standard storage is at 2-8 °C for short-term use, with the standard stock stored frozen at -20 °C to preserve antigen integrity across multiple freeze-thaw cycles. Researchers should aliquot reagents before freezing to minimize repeated freeze-thaw events.

The assay performs across the typical 8.0-5000 pg/mL dynamic range commonly reported for AFAP1 quantification, enabling reliable measurement of endogenous protein levels in cell lysates, tissue homogenates, and clinical specimens. The protocol is compatible with standard microplate readers and does not require specialized equipment beyond a 450 nm absorbance filter.