Human Advanced glycation end-product/AGE ELISA Kit

Assay Genie's Human Advanced Glycation End Product (AGE) ELISA Kit is designed for the quantitative detection of AGE in human samples. It uses a Sandwich ELISA format with a double antibody, with reactivity confirmed for human specimens and detection performed by colorimetric measurement at 450 nm. The kit is intended to support research into the role of AGEs in age-related conditions such as diabetes, Alzheimer's disease, and cardiovascular disease.

The kit reports a sensitivity of 0.188 ng/mL and a working range of 0.313-20 ng/mL. Compatible sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Storage is at 2-8°C for 12 months. Linearity in serum (n=5) was 86-100% at 1:2 dilution, 82-100% at 1:4, and 80-100% at 1:8. EDTA plasma (n=5) gave 85-100%, 82-94%, and 81-99% across the same dilutions, while heparin plasma (n=5) gave 85-102%, 89-93%, and 81-96%. Recovery averaged 94% for serum (range 88-97%), 95% for EDTA plasma (range 89-98%), and 98% for heparin plasma (range 88-105%).

The assay follows a six-step protocol: reagent and plate preparation; primary incubation at 37°C for 90 minutes to allow antigen binding; biotin-labelled detection antibody incubation at 37°C for 60 minutes; HRP-Streptavidin binding at 37°C for 30 minutes; TMB colour development in the dark for 10-20 minutes; and stop reaction with absorbance reading at 450 nm. The kit includes a pre-coated ELISA microplate, lyophilised standard, biotin-labelled antibody, HRP-Streptavidin conjugate, TMB substrate, sample dilution buffer, antibody dilution buffer, SABC dilution buffer, stop solution, wash buffer, plate sealer, and a technical manual.