Human Advanced glycation end-product (AGE) ELISA Kit

This sandwich ELISA from Assay Genie is designed to deliver accurate, reproducible quantification of human Advanced Glycation End Product (AGE) in biological samples. AGEs form through the non-enzymatic reaction of sugars with proteins, lipids, or nucleic acids, accumulating in tissues associated with diabetes, Alzheimer's disease, and cardiovascular conditions. The kit provides a straightforward workflow suitable for studying AGE biology and exploring disease mechanisms or potential therapeutic targets.

The colorimetric assay accepts serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, with signal read at 450 nm. Intended for research use only, the kit ships with a dismountable microplate, lyophilized standard, sample diluent, assay diluents A and B, detection reagents A and B, wash buffer, substrate, stop solution, and plate sealers.

Performance characteristics include a sensitivity of 0.41 ng/mL, a detection range of 0.78–50 ng/mL, an intra-assay CV of 4.2%, and an inter-assay CV of 6.6%. Dilutional linearity holds across 1:2 to 1:16 ratios in serum (86–116%), EDTA plasma (97–111%), and heparin plasma (85–111%). Mean recovery is 93% for serum (range 87–99%) and 95% for plasma (range 89–101%), and the assay recognises both natural and recombinant human AGE.