Human Adrenergic Receptor Alpha 2C (ADRa2C) ELISA Kit

Focused on the human alpha-2C adrenergic receptor (ADRA2C), a Gi/Go-coupled G protein-coupled receptor that modulates presynaptic catecholamine and norepinephrine release in the central and peripheral nervous system, this 96-test kit from Assay Genie enables quantitative receptor measurement in tissue homogenates and cell lysate preparations. ADRA2C is widely investigated in cardiovascular pharmacology, metabolic disease modeling, and neuropsychiatric research because its signaling axis governs sympathetic feedback inhibition.

Each plate ships pre-coated with a capture antibody directed against ADRA2C. Users add diluted samples alongside a recombinant receptor standard curve, incubate, and apply a labeled detection reagent. The enzymatic substrate reaction yields absorbance readings proportional to bound analyte, compatible with any standard microplate reader configured for colorimetric readout.

Unopened kits should be kept refrigerated; reconstituted components, opened vials, and aliquoted standards belong in a -20°C freezer, protected from light, until use. The reagents retain activity through the labeled shelf life when handled as recommended. Each box supplies the coated plate, assay buffer, wash concentrate, substrate solution, stop reagent, and materials for 96 individual tests.

Intended for in vitro research use only, the assay supports receptor biology, signal transduction, and drug target validation studies. Common uses include tracking ADRA2C expression changes following pharmacological intervention, gene editing, or disease model induction. Researchers applying the kit to platelet preparations, myocardial tissue, or defined brain regions should validate analyte recovery empirically, since performance in novel sample matrices can vary between laboratories.