Human ADORA1/Adenosine receptor A1 ELISA Kit

A high-quality sandwich ELISA from Assay Genie for the quantitative measurement of human ADORA1 (Adenosine receptor A1) levels. The colorimetric, double-antibody assay is reactive with human samples, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Linearity falls between 82% and 104% for serum, EDTA plasma, and heparin plasma across dilutions of 1:2, 1:4, and 1:8, and average recovery ranges from 93% to 96% across the same matrices. All reagents are intended for storage at 2–8°C for 12 months.

ADORA1 is a G-protein coupled receptor that mediates the physiological effects of adenosine, influencing neurotransmission, heart rate regulation, and inflammatory responses. Dysregulation has been implicated in neurodegenerative diseases, cardiovascular disorders, and inflammatory conditions, making it a target of interest in research and therapeutic development. The procedure includes a 90-minute primary incubation at 37°C, biotin-labelled detection antibody incubation, HRP-streptavidin binding, TMB colour development, and absorbance measurement at 450 nm.