Human ADH6 ELISA Kit (HUFI04669)

Built for laboratories measuring class V alcohol dehydrogenase activity in human tissues, this Assay Genie kit provides quantitative immunoassay reagents targeting the ADH6 enzyme. ADH6 contributes to cytosolic ethanol oxidation in hepatocytes and gastric mucosa, where it reduces a broad aldehyde substrate pool using NADH as a cofactor. Studies of hepatic enzyme induction, alcohol-metabolizing capacity, and aldehyde detoxification pathways use the kit to profile protein abundance across patient cohorts and matched controls.

The package supplies a pre-coated microplate, biotinylated detection reagents, streptavidin-enzyme conjugate, dilution buffer, wash concentrate, and a vial of calibrated standard for generating an eight-point curve. Researchers prepare samples by homogenizing snap-frozen liver biopsies, resuspending cultured hepatocyte pellets in lysis buffer, and clarifying lysates before loading. Recommended sample loading is 50 μL per well, with standards run in duplicate to anchor the calibration curve.

Reagent handling notes call for refrigerating unopened components, aliquoting the conjugate after first thaw, and limiting repeated freeze-thaw cycles to preserve binding activity. Diluted working solutions remain stable for short-term bench use within the manufacturer's stated shelf life. The assay supports downstream validation by western blot or immunohistochemistry on the same biological specimens, and the kit is labeled research use only.