Human Adaptor Related Protein Complex 2 Mu 1 (AP2m1) ELISA Kit

This Assay Genie microplate kit quantifies the mu (μ2) subunit of the heterotetrameric adaptor protein complex 2 (AP-2), a ~50 kDa component of the clathrin coat machinery that drives receptor-mediated endocytosis at the plasma membrane. AP2M1 encodes a medium subunit that recognizes tyrosine-based YXXφ sorting motifs on transmembrane cargo, recruiting receptors into clathrin-coated pits alongside the α, σ2, and β2 subunits.

Investigators studying membrane trafficking, synaptic vesicle recycling, and receptor internalization apply this assay to measure AP2m1 protein abundance in cell lysates and tissue homogenate preparations. Peer-reviewed applications include profiling AP-2 expression in neuronal disease models, characterizing adaptor redistribution after endocytic perturbation, and screening small molecules that disrupt clathrin-dependent uptake pathways. The reagents are supplied as a complete kit with one precoated strip plate, biotinylated detection antibody, streptavidin-HRP conjugate, lyophilized standards, wash buffer, and TMB substrate; refrigerate the unopened components and aliquot reconstituted standard to minimize freeze-thaw loss.