Human ADAMTS4 PharmaGenie ELISA Kit (SBRS0230)

Aggrecanase-1, encoded by the ADAMTS4 gene (A Disintegrin And Metalloproteinase with Thrombospondin motifs 4), is a zinc-dependent metalloproteinase that cleaves aggrecan, brevican, and other cartilage-matrix proteoglycans. Because of its central role in cartilage catabolism, ADAMTS4 is widely studied in osteoarthritis, rheumatoid arthritis, and intervertebral-disc degeneration research, and is also implicated in tumor invasion and inflammatory cytokine signaling.

Assay Genie provides this immunoassay as a 96-assay colorimetric ELISA configured for the in vitro quantitative measurement of human ADAMTS4 protein in research samples such as cell culture supernatant, plasma-derived fractions, and clotted serum specimens. The kit is intended for research use only and supports laboratories profiling cartilage-degrading enzyme activity, screening anti-inflammatory compounds, or monitoring ADAMTS4 expression in disease models. Each pack includes pre-coated microplate wells, calibrated standards, detection antibody, substrate, and the buffers needed to complete the workflow on a standard laboratory plate reader.

Researchers commonly pair this reagent with downstream western blot confirmation, cell-based aggrecanase activity assays, or quantitative PCR profiling of ADAMTS4 transcripts to corroborate observations across orthogonal readouts. Reconstituted reagents are typically aliquoted and stored to limit repeated freeze-thaw cycles, and lot-specific insert instructions should always be consulted for handling details.