Human ADAMTS18 ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit enables quantitative measurement of ADAMTS-18 in human samples. The assay uses a double-antibody sandwich ELISA format with colorimetric detection and accepts a wide range of liquid sample types including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

The kit has a reported sensitivity of 46.875 pg/mL and a measurable range of 78.125-5000 pg/mL. Linearity has been validated across serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions, with linearity values from 80% to 103%. Recovery ranges span 85-102% with averages between 92% and 95% depending on sample matrix. The procedure follows a standard sandwich ELISA workflow: reagent equilibration, sample incubation at 37°C for 90 minutes, biotin-labeled detection antibody incubation for 60 minutes, HRP-Streptavidin binding for 30 minutes, TMB substrate development in the dark for 10–20 minutes, and absorbance reading at 450 nm.

ADAMTS-18 (Uniprot Q8TE60) is an enzyme involved in extracellular matrix remodeling, with roles in tissue development, wound healing, and inflammatory responses. Dysregulation has been linked to cancer, arthritis, and cardiovascular disorders, making it a useful biomarker for disease and therapeutic research. Kit reagents are stored at 2-8°C for 12 months.