Human ADAMTS13 ELISA Kit

ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin type 1 motif, member 13) is a plasma zinc metalloprotease responsible for cleaving ultralarge von Willebrand factor multimers and restraining platelet-rich thrombus formation. Severely reduced activity is the established pathogenic mechanism behind thrombotic thrombocytopenic purpura (TTP), and quantitative measurement of the enzyme is therefore a routine target in hematology, vascular biology, and immunology research.

This immunoassay from Assay Genie is supplied in a microplate format and is configured for quantitative detection of human ADAMTS13 in laboratory research specimens. Reconstituted reagents and unused strips should be stored per the supplied protocol, and sealed components are typically held refrigerated to preserve activity through repeated freeze-thaw cycles. The assay is intended for research use only and is not cleared for diagnostic procedures.

A typical run begins with standards and samples incubated in pre-coated plate wells, followed by a biotinylated detection antibody and a streptavidin-HRP conjugate. Substrate development is stopped and absorbance is read against a standard curve to calculate sample values, supporting batch comparison of patient, control, and longitudinal specimens.