Human ADAM9 (A Disintegrin And Metalloprotease 9) ELISA Kit (HUES01502)

Researchers using this Assay Genie kit typically run the sandwich ELISA on a pre-coated 96-well microplate, add 100 µl of standards and diluted serum or plasma samples per well, and follow sequential incubation and wash steps before reading absorbance at 450 nm with a plate reader. The standard curve is usually constructed across a working range spanning roughly 0.1-10 ng/mL of recombinant ADAM9, allowing interpolation of unknown sample concentrations. Each kit provides a pre-coated plate, lyophilized protein standard, biotinylated detection antibody, streptavidin-HRP conjugate, TMB substrate, stop solution, and concentrated wash buffer sufficient for 96 determinations. ADAM9, also known as MDC9 or meltrin-gamma, is overexpressed in several solid tumor types and has been investigated as a candidate biomarker in prostate, pancreatic, and hepatocellular carcinoma research, as well as in inflammation and tissue remodeling models. Reconstituted standards should be stored frozen at -20°C in single-use aliquots to limit repeated freeze-thaw cycles, while unused microplate strips remain stable at 2-8°C in the resealed pouch with desiccant for the period indicated on the label. The assay is intended for in vitro research use only and supports relative quantification of ADAM9 in cell culture supernatants and tissue homogenates in addition to the standard serum and plasma matrices.