Human Acyl-CoA desaturase (SCD) ELISA Kit

This Sandwich ELISA kit from Assay Genie is designed for the quantitative measurement of human Acyl-CoA desaturase (SCD), and accepts sample types including serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The assay detects natural and recombinant human Acyl-CoA desaturase. Synonyms for the target include Delta(9)-desaturase, Fatty acid desaturase, Stearoyl-CoA desaturase, Delta-9 desaturase, and hSCD1.

Reported performance figures include a detection range of 0.156-10ng/mL and a sensitivity of 0.078ng/mL. Precision is characterised by an intra-assay CV of 5.7% and inter-assay CV of 7.0%. Linearity has been evaluated across serial dilutions from 1:2 to 1:16, in serum (85-97% to 106-116%), EDTA plasma (98-107% to 102-112%), and heparin plasma (88-98% to 114-123%). Recovery in serum averaged 102% (range 96-108%), and in plasma averaged 104% (range 98-110%).

Acyl-CoA desaturase is a stearyl-CoA desaturase that utilises O(2) and electrons from reduced cytochrome b5 to introduce the first cis double bond at the delta-9 position into saturated fatty acyl-CoA substrates, including palmitoyl-CoA and stearoyl-CoA, yielding a mixture of 16:1 and 18:1 unsaturated fatty acids. The enzyme contributes to lipid biosynthesis, regulation of lipogenic gene expression, body energy homeostasis, and the biosynthesis of membrane phospholipids, cholesterol esters and triglycerides. For research use only.