Human ACTL6A ELISA Kit (HUFI04680)

Built by Assay Genie for quantitative measurement of the BAF53a chromatin-remodeling subunit, this microplate ELISA delivers reproducible results in 3.5 hours when diluted serum, plasma, or cell lysate samples are run alongside a seven-point standard curve spanning 0.156–10 ng/mL. Researchers preparing serum should collect into a separator tube, allow clotting for 2 hours at room temperature or overnight, then centrifuge at 1000 × g for 20 minutes; plasma is collected with EDTA or heparin and spun at 1000 × g for 15 minutes at 2–8°C. Assay Genie recommends aliquoting cleared samples at -20°C or -80°C and avoiding repeated freeze-thaw cycles to preserve the 53 kDa BAF complex subunit. Unopened kit components are stored at 4°C, with the lyophilized standard kept at -20°C, giving a stated shelf life of 6 months from the date of receipt; the reconstituted standard at 10 ng/mL is suitable for use within a single working session. The capture antibody is human-specific, with no significant cross-reactivity reported against analogous actin-related proteins, supporting clean readouts in BRG1-associated factor tumor profiling and neural progenitor studies. The kit is supplied for research use only and is intended for in vitro applications.