Human Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

The Assay Genie absorbance-based immunoassay provides quantitative measurement of activated leukocyte cell adhesion molecule, also designated CD166, a 105 kDa transmembrane glycoprotein of the immunoglobulin superfamily implicated in immune cell migration, angiogenesis, and tumor progression studies. Researchers investigating stem-cell homing, neuroinflammation, and carcinoma metastasis apply this plate-based kit to monitor ALCAM expression across experimental conditions. The workflow accepts multiple specimen matrices and is calibrated for in vitro research use only.

The kit is supplied with reagents sufficient for 96 determinations on a pre-coated microplate. Components should be refrigerated upon receipt and brought to room temperature before running the protocol. Reconstituted standards and working dilutions are aliquoted for single use to limit repeated freeze-thaw cycles, which can degrade antibody performance in long-term storage.

Cell culture supernatants, tissue homogenates, and cell lysates are accepted input material alongside other clinical and experimental specimens, giving the workflow flexibility for animal-model work and patient-derived specimen banking. The substrate reaction produces a quantitative signal captured on a standard plate reader, supporting integration into high-throughput immunology pipelines. As with any antibody-based measurement, inclusion of appropriate positive and negative controls is recommended to confirm specificity in the chosen biological context.