Human Actinin Alpha 4 (ACTN4) ELISA Kit

Quantitative measurement of ACTN4, an actin-bundling protein that crosslinks actin filaments at focal adhesion sites and supports cytoskeletal reorganization during cell migration and mechanical stress response, is enabled by this Human Actinin Alpha 4 ELISA Kit from Assay Genie. ACTN4 plays a documented role in podocyte foot process maintenance, and dysregulated ACTN4 expression has been reported in focal segmental glomerulosclerosis as well as in invasive and metastatic phenotypes of several carcinoma cell lineages. Researchers working in nephrology, oncology, and cytoskeletal biology apply this kit to human tissue homogenates and cell lysates.

Configured for 96 assays, the kit ships as a complete package with pre-coated microplate wells, reference standard, detection reagents, substrate, and buffer solutions sufficient to generate a standard curve and analyze experimental samples in parallel. The workflow uses standard plate-reader signal acquisition following incubation and washing steps, and is intended for research use only in studies such as comparing ACTN4 levels between normal and transformed cells, monitoring knockdown or drug-treatment effects, or profiling expression across patient-derived tissue specimens.

For storage and handling, refrigerate kit components before first use, protect the substrate from light, and bring all reagents to room temperature prior to running the assay. Aliquot diluted working solutions to limit repeated freeze-thaw cycles, and treat the supplied wells as single use only.