Human Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit

The Assay Genie ACTA1 kit ships as a complete quantification platform featuring a pre-coated plate, a biotin-labeled capture antibody (100X concentrate), and an HRP-streptavidin conjugate (100X), plus lyophilized recombinant ACTa1 standards for curve generation. Each run uses 100 µL of standard or sample per well incubated at 37 °C, followed by sequential detection reagent incubations, plate washes, addition of 90 µL TMB substrate, and 50 µL stop solution before absorbance recording. The plate arrives as 8 x 12 removable strips sealed with desiccant in a foil pouch; unopened strips remain stable for 12 months when stored at -20 °C, and individual liquid components such as the biotinylated antibody, SABC, TMB substrate, and 25X wash buffer should be refrigerated and protected from direct light. Kit accessories include a plate sealer and a printed technical manual for step-by-step guidance.