Human ACTG1/Gamme Actin ELISA Kit

A sandwich ELISA kit from Assay Genie is designed for the quantitative detection of human gamma actin (ACTG1) in human samples. The assay uses a double-antibody sandwich format with colorimetric detection, and absorbance is read at 450 nm. Compatible sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and the kit is intended for cell biology research.

Performance specifications include a sensitivity of 18.75 pg/mL and a working range of 31.25–2000 pg/mL. The kit is stored at 2–8°C for 12 months. Listed synonyms for the target include ACTG1, Actin Gamma 1, ACTG, BRWS2, DFNA20, and DFNA26 (Uniprot P63261). Recovery in serum averages 98%, in EDTA plasma 99%, and in heparin plasma 100%.

Gamma actin is a cytoskeletal component essential for cell structure and function, and changes in its levels are relevant to research in cancer, cardiovascular disease, and neurological disorders. The kit provides a pre-coated microplate and a sample workflow that includes reagent equilibration, primary incubation at 37°C for 90 minutes, detection antibody binding, HRP-streptavidin binding, TMB color development, and a stop reaction.