Human ACTA1/Alpha Skeletal Muscle Actin ELISA Kit

From Assay Genie, this colorimetric sandwich ELISA is designed for the quantitative detection of human ACTA1 (alpha skeletal muscle actin) in research samples. The kit uses a double-antibody sandwich format and is validated for use with human serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types.

Reported analytical performance includes a sensitivity of 0.188 ng/mL and a working range of 0.313–20 ng/mL. Linearity has been assessed at 1:2, 1:4 and 1:8 dilutions in serum (88–103%, 86–105%, 90–102%), EDTA plasma (83–98%, 93–99%, 86–97%) and heparin plasma (81–96%, 85–96%, 84–100%), with average spike-and-recovery values of 96% for serum and 94% for both EDTA and heparin plasma. The target protein corresponds to UniProt P68133 and is described as playing a role in muscle contraction, with dysregulation linked to various muscle disorders, making it a biomarker of interest in muscle physiology and related disease research. Reagents are stored at 2–8°C for up to 12 months, with selected components suitable for longer-term storage at –20°C as described in the supplied manual.

The workflow follows a standard sandwich ELISA procedure: equilibrate reagents and TMB substrate, load standards, samples and controls onto the pre-coated plate and incubate at 37°C for 90 minutes for antigen binding; add biotin-labelled detection antibody and incubate at 37°C for 60 minutes; add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes; develop with TMB substrate in the dark for 10–20 minutes; then add stop solution and read absorbance at 450 nm.