Human ACSL5 ELISA Kit (HUFI04664)

ACSL5 (Acyl-CoA Synthetase Long Chain Family Member 5) is a long-chain fatty acid-CoA ligase that catalyzes the ATP-dependent activation of fatty acids to acyl-CoA thioesters, an essential step in cellular lipid biosynthesis and mitochondrial β-oxidation. Assay Genie's Human ACSL5 ELISA Kit provides a quantitative immunoassay for measuring endogenous ACSL5 protein in human research specimens, supporting studies of hepatic lipid handling, colorectal carcinoma biology, and disorders of fatty acid oxidation.

Validated for use with human serum and EDTA plasma specimens, the kit accommodates standard laboratory workflows with reagents sufficient for 96 individual determinations. Researchers have applied this assay to examine ACSL5 expression in hepatocellular carcinoma models, intestinal epithelial differentiation, and investigations of ER-mitochondrial membrane contacts in lipid droplet biogenesis.

Kit components include a pre-coated assay plate, biotinylated detection antibody, streptavidin-HRP conjugate, recombinant protein standards, and concentrated assay diluent. Store unopened reagents as indicated in the product insert and allow all components to equilibrate to room temperature before use. This product is intended for research use only and is not approved for diagnostic applications.