Human ACP3/PAP (Prostatic Acid Phosphatase 3) CLIA Kit (HUES01021)

Assay Genie provides a chemiluminescence immunoassay kit designed for the in vitro quantitative determination of Human ACP3/PAP (Prostatic Acid Phosphatase 3) in serum, plasma and other biological fluids. The assay uses a sandwich format with a run time of 4.5 hours. The microplate is pre-coated with antibody specific to Human ACP3/PAP, and detection relies on a biotinylated detection antibody with an Avidin-HRP conjugate; signal is read as Relative Light Unit (RLU) on a chemiluminescence analyzer.

Sensitivity is reported at 0.09 ng/mL with a detection range of 0.16–10 ng/mL, using a sample volume of 100 μL. The kit recognizes Human ACP3/PAP and shows no significant cross-reactivity or interference with analogues. Synonyms listed for the target include PSAP and ACPP, and the kit is intended for research in cell biology.

Reproducibility is stated as both intra-assay and inter-assay CV below 15%. Recovery testing across serum, EDTA plasma and cell culture media gave ranges of 95–109%, 92–104% and 90–105%, with average recoveries of 102%, 99% and 96% respectively.