Human ACK1/TNK2 ELISA Kit

A high-quality sandwich ELISA from Assay Genie designed for the accurate quantification of ACK1 (Activated Cdc42-associated kinase 1) and TNK2 (Tyrosine Kinase, non-receptor 2) levels in human samples. The assay has a sensitivity of 0.188 ng/mL and a detection range of 0.313–20 ng/mL, and is compatible with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

ACK1 and TNK2 are protein kinases involved in cellular processes including cell proliferation, survival, and migration, with dysregulation linked to cancer, autoimmune diseases, and other disorders. The kit supports research into these biomarkers and their potential as therapeutic targets. Reported linearity values (n=5) are 85–105%, 88–104%, and 89–98% for serum at 1:2, 1:4, and 1:8 dilutions; 83–100%, 88–100%, and 83–97% for EDTA plasma; and 82–98%, 81–94%, and 80–97% for heparin plasma. Recovery ranges are 91–101% for serum (96% average), 87–103% for EDTA plasma (96% average), and 85–102% for heparin plasma (96% average).

Storage is 2–8°C for 12 months. The detection method is colorimetric, using a biotin-labeled detection antibody, an HRP-streptavidin conjugate, and a TMB substrate, with absorbance read at 450 nm.