Human Acidic mammalian chitinase (CHIA) ELISA Kit

A high-quality assay from Assay Genie for quantitative measurement of human Acidic Mammalian Chitinase (CHIA), a glycosyl hydrolase 18 family enzyme that degrades chitin and chitotriose and is implicated in defence against fungal and nematode pathogens, T-helper cell type 2 (Th2) immune responses, and IL-13-mediated inflammation. The kit employs a Sandwich ELISA format and is intended for the detection of natural and recombinant human Acidic mammalian chitinase in human serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Quantitative performance characteristics supplied by the manufacturer include a detection range of 0.156-10 ng/mL and a sensitivity of 0.076 ng/mL. Reported precision values are an intra-assay CV of 5.8% and an inter-assay CV of 9.6%. Spike-and-recovery performance in serum and plasma is documented, and linearity has been evaluated in serum, EDTA plasma and heparin plasma at 1:2, 1:4, 1:8 and 1:16 dilutions. The protein corresponds to UniProt entry Q9BZP6, is located at chromosomal position 1p13.2, and the kit is intended for research use only within the Immunology research area.