Human Acetylcholinesterase (ACHE) ELISA Kit

A Sandwich ELISA kit from Assay Genie designed for the quantitative detection of human Acetylcholinesterase (ACHE), also known as AChE. The assay employs a colorimetric detection method and is intended for use with human samples including serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The manufacturer highlights the kit's high sensitivity and specificity for accurate and reproducible results.

The detection range is 0.312-20ng/mL with a sensitivity of 0.1ng/mL. Intra-assay precision is 6.1% CV and inter-assay precision is 10.2% CV. Linearity has been validated for serum, EDTA plasma and heparin plasma at dilutions of 1:2, 1:4, 1:8 and 1:16. Average recovery is 103% for serum (range 97-109%) and 105% for plasma (range 99-111%). The kit recognises natural and recombinant human Acetylcholinesterase and is for research use only within the neurosciences field. Acetylcholinesterase terminates signal transduction at the neuromuscular junction through rapid hydrolysis of acetylcholine in the synaptic cleft, and dysregulation of this enzyme has been linked to neurological disorders such as Alzheimer's disease and myasthenia gravis.