Human Acetyl-CoA acetyltransferase, mitochondrial (ACAT1) ELISA Kit

Assay Genie supplies a Sandwich ELISA kit for the quantitative detection of human Acetyl-CoA acetyltransferase, mitochondrial (ACAT1), a homotetramer located in the mitochondrion. The target is listed under synonyms including Acetoacetyl-CoA thiolase, T2, ACAT, and MAT, and is annotated to play a major role in ketone body metabolism. The kit is positioned within the cardiovascular research area.

Reported performance characteristics include a detection range of 7.8-500pg/mL, a sensitivity of 3.5pg/mL, an intra-assay CV of 4.2%, and an inter-assay CV of 9.2%. Linearity in serum (N=5) spans 89-99% at 1:2 to 94-107% at 1:16 dilution; EDTA plasma (N=5) ranges from 85-94% to 93-103% across the same dilutions, and heparin plasma (N=5) from 106-116% to 96-106%. Average recovery is 97% (91-103%) in serum and 99% (93-105%) in plasma.

The Assay Genie kit has been validated for use with serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, and shows specificity for natural and recombinant human Acetyl-CoA acetyltransferase, mitochondrial (UniProt P24752). The product is intended for research use only.