Human Absent In Melanoma 1 (AIM1) ELISA Kit

Quantitative ELISA kit from Assay Genie designed to measure Absent In Melanoma 1 (AIM1) protein levels in human research samples. AIM1 expression has been investigated in melanoma cell biology and in studies comparing tumor versus matched normal tissue, making this kit a useful tool for oncology and cell biology laboratories studying this protein.

Each kit contains the reagents and consumables required to perform 96 assays using a precoated microplate. The procedure follows a standard ELISA workflow with standard curve–based quantification, and the kit is validated for use with tissue homogenates and cell lysates. Researchers typically apply the assay to compare AIM1 levels across patient samples, screen cultured melanoma cell lines, or support downstream cell-based experiments in translational research.

Sample preparation generally involves homogenizing fresh or frozen tissue in a suitable lysis buffer, clarifying the lysate, and loading aliquots into the precoated wells. Components are supplied in individual vials with a detailed protocol insert outlining recommended dilutions and incubation times. To preserve reagent stability, keep kit components refrigerated between uses and protect from light until the assay is complete.