Human Abl Interactor 1 (ABI1) ELISA Kit

This 96-assay microplate immunoassay from Assay Genie enables quantitative measurement of human Abl Interactor 1 (ABI1) protein in research specimens. ABI1, also referred to as Abelson Interactor 1, E3B1, or NAP1, operates as a core component of the WAVE/SCAR regulatory complex that activates Arp2/3-mediated actin branching. The protein additionally serves as a substrate and binding partner for Abl family tyrosine kinases, linking cytoskeletal remodeling to kinase signaling in hematopoietic cells.

The kit is configured for in vitro research use and supports studies of cell migration, endocytosis, neuronal development, and leukemia-associated signaling pathways. Researchers apply it to compare ABI1 expression across treated and untreated samples, evaluate knockdown or overexpression experiments, and profile changes linked to Abl kinase activity. Matched antibody pairs are immobilized on a microplate, and detection relies on an enzyme-substrate reaction that produces a measurable signal read on a standard plate reader. Components are quality-controlled for batch consistency, and a standard curve of recombinant human ABI1 is included for accurate interpolation of unknown sample concentrations.

Unopened kits should be refrigerated upon receipt and equilibrated to room temperature before opening. Working solutions should be returned to refrigerated storage promptly after each run to preserve antibody and standard activity across repeated experiments. The Assay Genie Human Abl Interactor 1 (ABI1) ELISA Kit is supplied with a complete protocol, plate sealers, and sufficient reagents to complete the full 96-assay workflow.