Human ABCA1 (ATP-binding cassette transporter A1) CLIA Kit (HUES00371)

From Assay Genie, this CLIA Kit is designed for the in vitro quantitative determination of Human ABCA1 (ATP-binding cassette transporter A1) in serum, plasma and other biological fluids. The assay uses the Sandwich-CLIA principle with chemiluminescence detection, employing a pre-coated micro CLIA plate and a biotinylated detection antibody combined with an Avidin-Horseradish Peroxidase (HRP) conjugate. Relative light unit (RLU) values are read on a chemiluminescence immunoassay analyzer and are positively associated with Human ABCA1 concentration.

Reported performance characteristics include a sensitivity of 18.75 pg/mL, a detection range of 31.25-2000 pg/mL, a total assay time of 4.5h and a sample volume of 100 μL. Reproducibility is described as both intra-CV and inter-CV <15%. The kit recognizes Human ABCA1 in samples, with no significant cross-reactivity or interference between Human ABCA1 and analogues observed.

ABCA1 is a transporter involved in lipid transport and cholesterol homeostasis, making this assay relevant for research into cardiovascular disease, atherosclerosis, Alzheimer's disease and related areas of metabolism. Supplied kit components include reference standard, concentrated biotinylated detection antibody and HRP conjugate, diluents, wash buffer, substrate reagents, plate sealer and product documentation.