Human AAK1 (AP2-associated protein kinase 1) ELISA Kit (HUFI05483)

AAK1 (AP2-associated protein kinase 1) is a serine/threonine kinase that phosphorylates adaptor protein complex subunits AP-1 and AP-2, regulating clathrin-mediated endocytosis and cargo sorting at the plasma membrane. Dysregulated AAK1 activity has been linked to neurodevelopmental processes, viral entry mechanisms, and proliferative signalling in certain tumour models, making quantitative protein measurement a relevant tool in mechanistic cell biology research.

This immunoassay from Assay Genie provides a microplate-based workflow for quantifying human AAK1 in serum-derived specimens and matched plasma preparations. The 96 Assays kit ships with all required reagents in individual vials, calibrated plate strips, and a protocol insert intended for research use only in vitro applications. The absorbance-based readout is compatible with standard laboratory plate readers operating in the visible wavelength range.

Standard laboratory storage at refrigerated temperatures between experimental sessions preserves reagent activity through the kit's stated shelf life. Researchers investigating receptor internalisation, synaptic vesicle recycling, or chemokine receptor trafficking pathways commonly pair this ELISA with western blot and immunohistochemistry data in published studies of adaptor complex function.