Human A disintegrin and metalloproteinase with thrombospondin motifs 6 (ADAMTS6) ELISA Kit

This Sandwich ELISA Kit from Assay Genie is designed for the quantitative measurement of human A disintegrin and metalloproteinase with thrombospondin motifs 6 (ADAMTS6) in biological samples. ADAMTS6 is a metallopeptidase involved in extracellular matrix breakdown and tissue remodelling, with dysregulation implicated in cardiovascular diseases, arthritis, and certain cancers, making it a promising biomarker for studying these conditions. The kit detects natural and recombinant human ADAMTS6 and supports research in cell biology and protease-related disease pathology.

Validated for use with serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids, the kit employs a colorimetric detection method read at 450 nm. It offers a detection range of 31.2-2000pg/mL and a sensitivity of 10pg/ml. Reported precision is 6.9% intra-assay CV and 8.8% inter-assay CV. Linearity has been demonstrated in serum (97-120% across serial dilutions), EDTA plasma (80-120%), and heparin plasma (86-118%), with average recovery of 103% in serum and 105% in plasma.

For research use only, the kit targets the secreted ADAMTS6 protein (UniProt Q9UKP5; NCBI Gene ID 11174), which localises to the extracellular space and matrix. Researchers can apply it to investigate ADAMTS6 as a potential biomarker and to identify therapeutic targets in tissue remodelling and related disorders.