Human A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4) ELISA Kit

Aggrecanase-1 (ADAMTS4) is a zinc-dependent metalloproteinase that cleaves aggrecan core protein at specific glutamyl bonds, driving proteoglycan loss in articular cartilage. The enzyme's expression rises in osteoarthritis, rheumatoid arthritis synovium, and degenerating intervertebral discs, making it a translational biomarker for musculoskeletal disease activity.

This Assay Genie immunoassay delivers quantitative measurement of human ADAMTS4 across human serum samples, plasma samples, and tissue homogenate preparations drawn from joint, disc, or cartilage tissue sources. Each kit includes a pre-coated microplate, biotinylated detection reagent, streptavidin-HRP conjugate, chromogenic substrate, stop reagent, wash buffer, and recombinant human ADAMTS4 standards for curve generation.

The standard curve spans a working analytical range, allowing direct interpolation of unknowns with minimal dilution. The chromogenic endpoint is read on a microplate spectrophotometer, with signal intensity proportional to enzyme concentration. Researchers apply this kit to chondrocyte stimulation experiments, cartilage explant culture studies, synovial fluid biomarker profiling, and in vitro drug screening for matrix-protective compounds.

Reconstituted standards and diluted samples are aliquoted and stored at -20°C to -80°C for repeated use, while working dilutions of detection antibody should be kept refrigerated at 4°C during the assay workflow. The microplate can be resealed with desiccant for short-term storage per the package insert. This format supports longitudinal studies and paired-sample comparisons across cartilage biology, rheumatology, and orthopaedic research programs.