Human A Disintegrin And Metalloprotease 33 (ADAM33) ELISA Kit

This quantitative immunoassay from Assay Genie measures human A Disintegrin And Metalloprotease 33 (ADAM33) protein in research samples, supporting investigations into asthma susceptibility, airway hyperresponsiveness, and respiratory tissue remodeling. ADAM33 is a type I transmembrane metalloprotease predominantly expressed by mesenchymal cells such as fibroblasts and airway smooth muscle cells, where it mediates ectodomain shedding of growth factors including TGF-α, HB-EGF, and amphiregulin, and contributes to cell-cell and cell-matrix adhesion through its disintegrin domain.

The kit accommodates tissue homogenates and cell lysates, making it applicable to studies using bronchial biopsy material, cultured airway smooth muscle cells, or fibroblast lysates from lung tissue. Researchers examining Th2-high asthma phenotypes, epithelial-mesenchymal signaling, or bronchoconstriction pathways can use this assay to quantify ADAM33 expression or shed-domain release across patient cohorts and model systems.

Each kit provides reagents sufficient for 96 assays, with components supplied in a ready-to-use format that minimizes hands-on preparation. The protocol is compatible with standard plate-based absorbance reading, allowing integration into existing laboratory workflows for respiratory biology, pharmacology, and biomarker discovery research. Reagents should be refrigerated upon receipt and protected from light until use.