Human 5-oxoprolinase (OPLAH) ELISA Kit

A high-quality sandwich ELISA from Assay Genie designed for the quantitative detection of human 5-oxoprolinase (OPLAH), an enzyme involved in the gamma-glutamyl cycle that plays a critical role in glutathione metabolism. The assay is reactive with human samples and is suitable for use with serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Reported performance characteristics include a detection range of 0.156-10 ng/mL and a sensitivity of 0.076 ng/mL, with an intra-assay CV of 5.6% and an inter-assay CV of 9.4%. The kit is specific for natural and recombinant human 5-oxoprolinase, and OPLAH functions as a homodimer that catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate. Research applications fall within cell biology, and dysregulation of 5-oxoprolinase has been implicated in metabolic disorders and neurodegenerative conditions. For research use only.