Human 5'-Nucleotidase, Ecto (NT5E) ELISA Kit

Built around ecto-5'-nucleotidase (CD73), a glycosylphosphatidylinositol-anchored surface enzyme that converts extracellular AMP into adenosine, this colorimetric ELISA Kit from Assay Genie enables researchers to quantify human NT5E across blood-derived fractions, tissue homogenates, cell lysates, and conditioned media from in vitro cell culture. CD73 sits at a pivotal node in purinergic signaling, shaping tumor microenvironment immunosuppression, endothelial barrier function, and lymphocyte regulation, which makes it a frequent target in oncology, fibrosis, and inflammatory disease research.

Supplied as a complete kit configured for 96 reactions, the assay provides all reagents needed for that number of individual determinations on a single plate. Validated for human samples and intended for research use only, the kit supports studies that treat NT5E as a candidate biomarker, including work on immunotherapy response, adenosine pathway activity, and T-cell subset behavior.