Human 5-hydroxytryptamine receptor 1B (HTR1B) ELISA Kit

From Assay Genie, this Sandwich ELISA is designed for the precise detection of 5-hydroxytryptamine receptor 1B (HTR1B) levels in human serum, plasma, and cell culture supernatants. The kit provides reactivity specific to human samples and offers a sensitivity of 34pg/mL across a detection range of 78-5000pg/mL. Intra-assay precision is 4.6% (CV) and inter-assay precision is 8.5% (CV). Recovery in serum averages 97% (range 91-103%) and in plasma 99% (range 93-105%). Specificity is directed against natural and recombinant human HTR1B.

5-hydroxytryptamine receptor 1B is a G-protein coupled receptor for serotonin that regulates the release of 5-hydroxytryptamine, dopamine and acetylcholine in the brain, thereby affecting neural activity, nociceptive processing, pain perception, mood and behavior. The receptor also plays a role in vasoconstriction of cerebral arteries. Dysregulation of HTR1B has been linked to various neurological and psychiatric disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions. The protein localises to the cell membrane as a multi-pass membrane protein (UniProt P28222, 43,568 Da) and falls within the Neurosciences research area.

The kit is intended for research use only. Supplied components include a dismountable ELISA microplate, lyophilised standard, sample diluent, assay diluents, detection reagents, wash buffer, substrate solution, stop solution and plate sealer. Full assay procedure and storage details are provided with the kit.