Human 4-Hydroxyphenylpyruvate Dioxygenase (HPD) ELISA Kit

The Assay Genie Human 4-Hydroxyphenylpyruvate Dioxygenase (HPD) ELISA Kit enables quantitative measurement of HPD, a non-heme iron-dependent dioxygenase that catalyzes the second step of tyrosine catabolism, converting 4-hydroxyphenylpyruvate to homogentisate primarily in hepatic tissue.

HPD is clinically significant because biallelic loss-of-function variants in the HPD gene cause Tyrosinemia Type III, an autosomal recessive metabolic disorder characterized by accumulation of 4-hydroxyphenylpyruvate and 4-hydroxyphenyllactate. Investigators working on inborn errors of metabolism, hepatic enzyme expression, and the phenylalanine-tyrosine degradation pathway use this ELISA to quantify HPD protein across patient samples and experimental models.

The kit is supplied as a pre-coated microplate and is intended for in vitro research use only. Reagents should be refrigerated upon arrival and allowed to equilibrate to room temperature prior to running the assay. Each pack contains reagents sufficient for 96 determinations in a single-use plate format.