Human 3-oxo-5-alpha-steroid 4-dehydrogenase 1 (SRD5A1) ELISA Kit

A high-quality Sandwich ELISA from Assay Genie designed for the precise quantification of human 3-oxo-5-alpha-steroid 4-dehydrogenase 1 (SRD5A1) levels in human serum, plasma, and cell culture supernatants. The assay also accepts tissue homogenates and other biological fluids, with specificity for natural and recombinant human SRD5A1. Reported performance figures include a detection range of 0.156–10 ng/ml, a sensitivity of 0.078 ng/mL, an intra-assay CV of 6.7% and an inter-assay CV of 10.2%.

SRD5A1, also known as steroid 5 alpha-reductase 1, is the enzyme that converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids, playing a central role in sexual differentiation and androgen physiology. The kit is intended for research use only, with applications including studies in neurosciences. The target protein is localised to the microsome membrane and endoplasmic reticulum membrane as a multi-pass membrane protein.