Human 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) ELISA Kit

This quantitative immunoassay from Assay Genie measures human 17β-Hydroxysteroid Dehydrogenase Type 3, a microsomal NADPH-dependent oxidoreductase that catalyzes the conversion of androstenedione to testosterone in testicular Leydig cells. Because HSD17b3 is central to male androgen biosynthesis, researchers apply the kit to studies of reproductive endocrinology, 46,XY disorders of sex development, and gonadal steroid metabolism.

The plate-based configuration processes multiple samples per run and supplies the buffers, capture antibody, detection antibody, and substrate required to complete the protocol. Reconstituted standards and detection reagents are stored refrigerated and protected from light; each antibody vial is intended for single use. To preserve analyte integrity, blood-derived samples and tissue lysates are aliquoted after collection and held at -80 °C, with repeated freeze-thaw cycles avoided.

HSD17b3 measurement is frequently paired with orthogonal readouts such as western blot, immunohistochemistry on testicular sections, and PCR profiling of steroidogenic transcripts, giving laboratories a multi-layered view of androgen biosynthesis pathways.