Human 17-beta-hydroxysteroid dehydrogenase 13 (HSD17B13) ELISA Kit

Assay Genie offers this colorimetric Sandwich ELISA for the quantitative measurement of human 17-beta-hydroxysteroid dehydrogenase 13 (HSD17B13) in human serum, plasma, cell culture supernatants, tissue homogenates, and other biological fluids. The kit is intended for research use only.

HSD17B13 is a member of the short-chain dehydrogenases/reductases (SDR) family and has been linked to lipid metabolism, liver diseases, metabolic disorders, and obesity. The assay is specific for natural and recombinant human HSD17B13 and is relevant to signal transduction research. Reported performance characteristics include a detection range of 0.312–20 ng/mL, a sensitivity of 0.156 ng/mL, an intra-assay CV of 8.4%, and an inter-assay CV of 11.1%. Linearity has been demonstrated in serum, EDTA plasma, and heparin plasma across dilutions of 1:2, 1:4, 1:8, and 1:16, with reported serum recovery averaging 87% (range 81–93%) and plasma recovery averaging 89% (range 83–95%). The associated UniProt entry is Q7Z5P4.

Detection follows a standard sandwich ELISA workflow with absorbance measured at 450 nm. The kit provides a dismountable microplate, lyophilized standard, sample and assay diluents, detection reagents, wash buffer, substrate, stop solution, and plate sealer, with storage temperatures specified per component in the kit insert.