Human 14-3-3 protein theta/YWHAQ ELISA Kit

This Sandwich ELISA kit from Assay Genie is designed for the quantitative measurement of human 14-3-3 protein theta (YWHAQ), an adaptor protein involved in signal transduction, cell cycle regulation, and apoptosis. The kit uses a colorimetric detection method and is reactive with human samples, including serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. Dysregulation of YWHAQ has been linked in the literature to diseases such as cancer, Alzheimer's, and Parkinson's, supporting its relevance as a research biomarker.

The assay has a detection range of 0.78–50 ng/mL and a sensitivity of 0.43 ng/mL. Precision values are 4.3% intra-assay CV and 7.8% inter-assay CV. Linearity has been validated in serum, EDTA plasma, and heparin plasma across 1:2 to 1:16 dilutions, with recovery of 82–94% in serum and 84–96% in plasma. Specificity is confirmed against natural and recombinant human 14-3-3 protein theta.