Human 12-LO/ALOX12 ELISA Kit

From Assay Genie, this Sandwich ELISA kit provides a quantitative tool for measuring human 12-lipoxygenase (ALOX12) levels in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Using a double-antibody sandwich format with colorimetric detection, the kit offers a sensitivity of 0.188 ng/mL and a standard range of 0.313–20 ng/mL.

12-lipoxygenase (ALOX12) is an enzyme involved in the metabolism of arachidonic acid and plays a role in inflammation and oxidative stress; its dysregulation has been linked to asthma, atherosclerosis, and cancer. Recovery in serum averaged 98% (range 92–103%), in EDTA plasma 95% (range 90–101%), and in heparin plasma 94% (range 88–97%). All components are stored at 2–8°C for 12 months.