Human 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) ELISA Kit

The HSD11B2 enzyme governs local glucocorticoid activity by converting active cortisol to inactive cortisone in mineralocorticoid-responsive tissues. This quantitative immunoassay kit from Assay Genie supports research on hypertension, preeclampsia, placental glucocorticoid barrier function, and altered cortisol metabolism linked to metabolic and cardiovascular disease.

Validated specifically for human samples with no significant cross-reactivity observed against other species evaluated during assay development, the kit enables targeted measurement of HSD11B2 protein in tissue homogenates and cell lysates prepared from experimental sources. Each pack includes a pre-coated microplate, lyophilized standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash and dilution buffers, chromogenic substrate, and stop reagent sufficient for 96 individual sample determinations in a microplate format.

The workflow follows standard steps: standards and samples are added to coated wells, followed by incubation with detection antibody and HRP conjugate, then substrate for absorbance-based readout. Unopened kit components should be stored refrigerated, with working dilutions prepared immediately before use. Each kit is intended for research use only and supports studies ranging from basic enzyme characterization through to disease mechanism investigation.