Human 10 kDa heat shock protein, mitochondrial (HSPE1) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is a colorimetric assay designed for the quantitative determination of human 10 kDa heat shock protein, mitochondrial (HSPE1) in human samples, including serum and plasma. The kit specifically detects natural and recombinant human HSPE1, supporting research into mitochondrial dysfunction and cellular stress response.

Performance characteristics include a detection range of 3.12-200ng/mL and a sensitivity of 1.72ng/mL. Reported precision is 6.6% intra-assay CV and 6.5% inter-assay CV. Linearity in serum (N=5) ranges from 96-106% at 1:2 to 100-110% at 1:16, with EDTA plasma showing 93-103% to 93-105% and heparin plasma showing 112-122% to 107-117% across the same dilutions. Recovery in serum averages 100% (range 94-106%) and in plasma averages 102% (range 96-108%).

HSPE1 functions as a mitochondrial co-chaperonin that, together with Hsp60, facilitates the correct folding of imported proteins and may prevent misfolding under stress conditions in the mitochondrial matrix. The kit is intended for research use only and is suitable for serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids.